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Transporting nLs, uLs and pLs To Targets, Non-touch, For Many Purposes With IBF.

 (Course Outline Below*.)

 

Honorable Mention: Best new technology: Pittcon 2007 & 2008 ! 

 

2007/08 selected attendees: students from Los Alamos, Northrup, Foss, LLNL, Battelle, NIST, Applied Bio, PNL, Idexcorp, NIH, Spark Holland, Lumera, Lee Co. and major U's.   

Nanoliter LLC's Mr. Drew Sauter, Dr. M. Johnson of Duquesne U. and Dr. J. Harmon of U. of South Florida will present the 4 hour course at Pittcon 2008.  Call for special courses at Nanoliter,LLC.

PMMA graph at far left, from U. South Florida: B. Hilker, Kevin Clifford & J. Harmon using Nanoliter Wave nanoliter syringe deposition system of PMMA polymer. 100% Sweet spot?.

 

 

Extend the ability of your facility to routinely handle liquids by three orders of magnitude at low cost with very little training.

Also, fly nLs, uLs of liquids to MALDI plates, students, food, microscope slides, vials, mtp and more in milliseconds.

Launch glycerin, slurries, glues; serum and other difficult samples to targets, non-touch.

Learn theory and practical aspects of MALDI,  polymer and other sample preparation characterizations.

See nL, IBF enhanced Pipettes & Pumps.

Learn how to count nanoliters!

 

Dear Colleague:

 

We are pleased to announce our course entitled:" Transporting nLs, uLs and pLs To Targets, Non-touch, For Many Purposes With IBF." This technology which received an honorable mention at Pittcon 2007 and 2008 for best new instrument offers new ways to handle and prepare sample.  Just as energized gas phase ions in mass spectrometers can be directed to fly to targets across from um to m, the same is true for liquids energized appropriately. This property allows one to dispense nanoliters, microliters and picoliters without touch, flying them with direction to and from common targets, even straight up as well show in the course. In the course, we present practical examples and applications of handling nLs, uLs and pLs. We address the chemistry and the physics of  IBF briefly contrasting it to ESI and traditional microfluidics, as we forward technical applications and opportunities that result from what amount to a complete new TOOL SET for liquid handling that anyone can employ and learn in literally minutes. We include in the course an overview of micro and macro fluidics so one can contrast old and new technologies.

 

Our course employs videos and demos as away to covey IBF technology including:

 

    the nanoliter, microliter syringe demo,

    parallel LC with tight, regular sample placement directly from the monolithic LC columns which can significantly increase MALDI sensitivity;

    the  nanoliter pipette,

    morphed nanoliter syringe pumps and peristaltic pumps;

    48 channel dispensings; the creation and aspiration of nanoliter-sicles;

    nanoliter dispensing of glycerin and other problem liquids;

    nanoliter drug delivery into humans;

    nanoliters flying into microtiter plates;

    nanoliters and microliters flying to and from/to humans..

    and new applications.

 

IBF allows you to begin to do completely new tasks, and it can inexpensively extend the ability of your facility to routinely handle liquids by three orders of magnitude! Of course, it therefore allows you to save 10, 100 to 1000 X on the cost of reagents, saving precious reagents/samples, as you reduce waste disposal and human exposure by large factors, as you are otherwise empowered..

 

Uniquely, the course is designed for scientists, engineers, scientific instrument firms, pump manufacturers, fluidics firms and analytical chemist. Those involved in  mass spectroscopy, microscopy, homeland security, forensics, Lab Chips, fluidics expendables, drug development and biotechnology firms will also benefit as well. The course will be taught by Dr. Mitch Johnson  of Duquesne University who has been working in microfluidics at the University of Virginia and whose talents in microfluidics, chromatography, electrophoresis, LIF complement those of Mr. Drew Sauter, the inventor of IBF.  Both are early adopters of new technology in GC/MS, LC/MS, MS/MS, macro, microfluidics and now induction based fluidics, (IBF).  To request a course schedule contact us at nanoliter.com or call 702-896-5413.

 

 Thank you, in advance, for your consideration and time.

 

Sincerely yours,

Mr. Drew Sauter

Nanoliter

Henderson, NV  702-896-5413

 

Dr. Mitch Johnson

Duquesne University,

Pittsburgh, PA, 412-396-5278.

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Transporting nLs, uLs and pLs To Targets, Non-touch, From & To Entities & Devices Of Many Types, For Many Purposes With IBF.

 

 

Course  Outline 

This outline is more does not include Dr. Johnson's (microfluidics)  and Dr. Harmon's (polymer, MALDI and  newer apps.) contributions.

 

Introduction

Why nanoliters and IBF?. Economics, health, aesthetics & practicality

Traditional/non-traditional fluidic devices considered  

Limitations of pipettes, syringes, mechanical systems

IBF history

                Studying ESI w/Agilent, Affymax and Chem Space to understand ESI

                                Traditional microfluidics contrasted to ESI

                                ESI contrasted to IBF

                                                Other liquid movement approaches addressed and contrasted.

    e.g., Millikan,inkjets; piezo, chips.

 

Example IBF Applications with Video.

Parallel dispensing, parallel LC with tight MALDI sample placement

Million fold dilution, e-spotting, TLC, toxicology chemicals onto animals, NCB sample handling

DNA/RNA prep. Samples fly to humans for dispensing medication.  Others

Demo of the nl/uL Syringe.

 

Moving matter.

              Laminar  flow, capillary action, adhesion and cohesion.

                              Flying matter

    Baseballs, Bullets, Electrons in tubes, Millikan's experiment, Inkjets

              IBF and Mass Spectrometers {ions, gasses, liquids}

                                Forces considered-contrasted. Liquid trajectories calculated

Explaining energy of flying nanoliters in one application

                            Simple explanation of IBF in a capillary

Simple explanation of IBF flying liquids to humans

Simple explanation of parallel IBF dispensing
               

Instrumentation: The Nanoliter Syringe, The Nanoliter Pipette, The Nanoliter Pumps

                Liquid transport specification (Simple or complex task.)

                Plumbing, manifold or chip considerations

                Energy sources

Electrokinetic

Pneumatic, peristaltic, ultrasonic, other for hybrid systems

Robotic considerations

Interface/s

Detection

 

Practical issues in liquid transport, across the macro, micro and nano regimes.

Evaporation after Maxwell

Electrochemistry after Adams/Bard.

Particles

Viscosity

Surfaces Energy

Joule heating

Environmental factors

Non ideal consideration in nanofluidics briefly considered

 

Calibration

               Approaches

Photoshop/Paintshop Pro demo

               Calibration software demo

               Accuracy & precision estimates for various modes/devices.

               Newer approaches

Discussion and cautions.

 

Other IBF based liquid movement techniques, outside the box.

                New Applications of IBF to Polymer Characterizations.

                Nanoliter-sicles, making frozen charged spheres of liquid/s

Writing the word "picoliters" with pLs and IBF. The picoliter regime

The New Nanoliter/Microliter syringe (Patent Pending.)

Printing with monolithic LC columns for MALDI sample preparation

More morphing syringe pumps, peristaltic and other pumps into the nanoliter regime

Passive/active focusing. 

 

Summary.

            Why, where having the ability to manipulate nanoliters at your locale has merit.

Competitive techniques

Questions and answers

 

Handouts. Course Book, Article Reprints, Nanoliters

 

We reserve the right to modify the course as we solely determine. 

 

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