Nanoliters: Fundamentals Of Induction Based Fluidics.
Applications To Sample Preparation/Treatment & Instrument Introduction.
To TLC, MALDI, SPE, IR, LC, SIMS, UV/Vis, ESI,
DART & More.
Introduction
Why nanoliters and IBF?. Economics, health, aesthetics & practicality
Traditional/non-traditional fluidic devices considered
Limitations of pipettes, syringes, mechanical systems
IBF history
Studying ESI w/Agilent, Affymax and Chem Space to understand ESI
Traditional microfluidics contrasted to ESI
ESI contrasted to IBF
Other liquid movement approaches addressed and contrasted.
e.g., Millikan,inkjets; piezo, chips.
Example IBF Applications with Video.
Parallel dispensing, parallel LC with tight MALDI sample placement
Million fold dilution, e-spotting, TLC, toxicology chemicals onto animals, NCB sample handling
DNA/RNA prep. Samples fly to humans for dispensing medication. Others
Demo of the nl/uL Syringe.
Moving matter.
Laminar flow, capillary action, adhesion and cohesion.
Flying matter
Baseballs, Bullets, Electrons in tubes, Millikan's experiment, Inkjets
IBF and Mass Spectrometers {ions, gasses, liquids}
Forces considered-contrasted. Liquid trajectories calculated
Explaining energy of flying nanoliters in one application
Simple explanation of IBF in a capillary
Simple explanation of IBF flying liquids to humans
Simple explanation of parallel IBF
dispensing
Home
Instrumentation: The Nanoliter Syringe, The Nanoliter Pipette, The Nanoliter Pumps
Liquid transport specification (Simple or complex task.)
Plumbing, manifold or chip considerations
Energy sources
Electrokinetic
Pneumatic, peristaltic, ultrasonic, other for hybrid systems
Robotic considerations
Interface/s
Detection
Practical issues in liquid transport, across the macro, micro and nano regimes.
Evaporation after Maxwell
Electrochemistry after Adams/Bard.
Particles
Viscosity
Surfaces Energy
Joule heating
Environmental factors
Non ideal consideration in nanofluidics briefly considered
New Publications.
Calibration
Approaches
Photoshop/Paintshop Pro demo
Calibration software demo
Accuracy & precision estimates for various modes/devices.
Newer approaches
Discussion and cautions.
Other IBF based liquid movement techniques, outside the box.
Nanoliter-sicles, making frozen charged spheres of liquid/s
Writing the word "picoliters" with pLs and IBF. The picoliter regime
The New Nanoliter/Microliter syringe (Patent Pending.)
Printing with monolithic LC columns for MALDI sample preparation
More morphing syringe pumps, peristaltic and other pumps into the nanoliter regime
Passive/active focusing.
Summary.
Why, where having the ability to manipulate nanoliters at your locale has merit.
Competitive techniques
Questions and answers
Handouts. Course Book, Article Reprints, Nanoliters
Course outline can be updated as and when we determine.